Table 2.
Cytokine levels 3 dpi in lung samples from mice challenged with aerosolized Y. pestis C12, stratified by sex and by high (> 106 CFU/g) or low (< 103 CFU/g) bacterial burden.
| Females | Males | Females | Males | |||||
|---|---|---|---|---|---|---|---|---|
| Yp low* | Yp high* | Yp low* | Yp high* | hi/lo ratio | p-value ** | hi/lo ratio | p-value ** | |
| Yp CFU/g | 1.39E+02 (4.39E+01) | 6.16E+08 (1.18E+08) | 1.22E+02 (5.89E+01) | 9.59E+08 (1.18E+08) | ||||
| IL-6 | 28.3 (4.1) | 12,559.6 (1,553.7) | 23.9 (2.2) | 13,136.7 (1,625.2) | 443.29 | 2.91E-05 | 548.77 | 4.28E-04 |
| G-CSF | 3.0 (0.6) | 262.6 (17.2) | 1.8 (0.2) | 290.8 (21.0) | 88.49 | 2.90E-05 | 157.80 | 4.24E-04 |
| IFN-γ | 11.8 (3.6) | 635.9 (87.7) | 6.6 (1.6) | 598.6 (89.7) | 53.67 | 2.92E-05 | 90.92 | 4.28E-04 |
| IL-1β | 12.9 (3.1) | 607.6 (64.1) | 9.4 (1.8) | 551.4 (34.4) | 47.20 | 2.92E-05 | 58.46 | 4.28E-04 |
| IL-1α | 17.1 (2.1) | 574.7 (102.7) | 17.9 (3.1) | 359.5 (38.7) | 33.58 | 2.92E-05 | 20.04 | 4.28E-04 |
| LIF | 19.0 (4.3) | 593.6 (30.4) | 8.3 (1.6) | 590.9 (23.5) | 31.31 | 2.92E-05 | 71.30 | 4.28E-04 |
| IL-22 | 43.4 (6.6) | 1,118.3 (98.5) | 31.3 (6.3) | 653.8 (62.9) | 25.76 | 2.92E-05 | 20.91 | 4.28E-04 |
| MIP-1α (CCL3) | 18.3 (3.9) | 463.1 (68.5) | 8.5 (1.1) | 499.9 (28.7) | 25.36 | 2.92E-05 | 58.73 | 4.28E-04 |
| GM-CSF | 7.1 (1.1) | 171.5 (14.1) | 4.0 (0.6) | 172.7 (15.5) | 24.21 | 2.92E-05 | 42.91 | 4.28E-04 |
| GRO-α (CXCL1) | 138.9 (42.2) | 3,275.6 (275.6) | 58.8 (19.5) | 4,213.2 (250.1) | 23.59 | 2.91E-05 | 71.63 | 4.21E-04 |
| TNF-α | 4.1 (0.6) | 90.4 (8.2) | 3.2 (0.3) | 94.2 (5.2) | 21.80 | 2.90E-05 | 29.46 | 4.27E-04 |
| IL-17A (CTLA-8) | 61.1 (26.3) | 664.7 (36.3) | 22.9 (8.3) | 684.2 (30.9) | 10.88 | 3.51E-05 | 29.81 | 4.28E-04 |
| MIP-1β (CCL4) | 36.2 (8.5) | 363.4 (50.1) | 15.4 (2.8) | 509.5 (47.6) | 10.04 | 2.91E-05 | 33.15 | 4.28E-04 |
| M-CSF | 1.7 (0.4) | 6.9 (0.9) | 2.1 (0.7) | 6.2 (0.9) | 4.06 | 1.02E-04 | 2.91 | 0.022 |
| IL-27 | 6.1 (1.1) | 20.7 (2.4) | 2.5 (0.2) | 17.4 (1.4) | 3.41 | 2.37E-04 | 6.88 | 4.28E-04 |
| IL-12p70 | 6.6 (0.7) | 17.4 (1.5) | 4.6 (0.7) | 14.8 (1.3) | 2.64 | 3.29E-04 | 3.21 | 0.002 |
| ENA-78 (CXCL5) | 78.5 (13.1) | 182.0 (21.6) | 101.1 (25.0) | 159.1 (18.3) | 2.32 | 0.006 | 1.57 | 0.109 |
| IP-10 (CXCL10) | 99.2 (17.9) | 219.9 (21.3) | 60.8 (11.3) | 263.6 (16.8) | 2.22 | 3.29E-04 | 4.34 | 4.28E-04 |
| MCP-3 (CCL7) | 134.6 (17.6) | 283.1 (28.3) | 104.3 (19.1) | 364.0 (38.7) | 2.10 | 6.03E-05 | 3.49 | 4.28E-04 |
| IL-9 | 28.6 (3.1) | 57.4 (3.3) | 25.5 (2.3) | 48.9 (2.8) | 2.01 | 1.02E-04 | 1.92 | 0.002 |
| IL-15 | 9.2 (0.6) | 15.6 (1.1) | 6.9 (0.9) | 13.6 (1.0) | 1.68 | 0.002 | 1.95 | 0.004 |
| IL-10 | 15.3 (1.3) | 24.3 (1.9) | 13.3 (1.0) | 23.4 (2.4) | 1.59 | 0.003 | 1.75 | 0.032 |
| IL-4 | 11.9 (1.9) | 17.7 (4.3) | 4.6 (0.6) | 9.7 (1.5) | 1.49 | 0.983 | 2.11 | 0.143 |
| IL-2 | 3.8 (0.3) | 5.0 (0.3) | 3.6 (0.2) | 5.1 (0.5) | 1.32 | 0.027 | 1.40 | 0.272 |
| RANTES (CCL5) | 438.3 (12.7) | 415.5 (24.8) | 320.8 (34.5) | 403.8 (23.6) | 0.95 | 0.413 | 1.26 | 0.100 |
| IL-5 | 59.4 (15.1) | 48.9 (9.8) | 10.1 (5.2) | 38.7 (6.9) | 0.82 | 0.345 | 3.82 | 0.015 |
| Eotaxin | 517.1 (60.9) | 399.5 (41.5) | 441.7 (92.1) | 455.5 (35.9) | 0.77 | 0.033 | 1.03 | 1.000 |
| IL-23 | 23.2 (3.8) | 16.2 (1.2) | 21.3 (2.0) | 11.3 (0.6) | 0.70 | 0.022 | 0.53 | 7.05E-04 |
| IL-28 | 83.8 (11.0) | 46.3 (2.9) | 78.8 (13.0) | 40.5 (0.7) | 0.55 | 0.002 | 0.51 | 2.42E-04 |
| IL-13 | 36.7 (10.9) | 19.7 (1.6) | 6.6 (1.8) | 15.1 (1.1) | 0.54 | 0.062 | 2.29 | 0.005 |
| sample size | 8 | 25 | 5 | 31 |
|
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*All values shown as mean (SEM).
**Mann-Whitney test for comparison of high-burden to low-burden samples within the same sex.
Bolded, p < 0.05.