Table 3.
Cytokine levels 3 dpi in spleen samples from mice challenged with aerosolized Y. pestis C12, stratified by sex and by high (> 8x103 CFU/g) or low (< 2x103 CFU/g) bacterial burden.
| Females | Males | Females | Males | |||||
|---|---|---|---|---|---|---|---|---|
| Yp low* | Yp high* | Yp low* | Yp high* | hi/lo ratio | p-value ** | hi/lo ratio | p-value ** | |
| Yp CFU/g | 3.12E+01 (2.42E+01) | 1.53E+08 (1.05E+08) | 3.14E+02 (1.60E+02) | 5.05E+08 (2.61E+08) | ||||
| IFN-γ | 2.4 (0.2) | 838.1 (178.9) | 2.2 (0.4) | 478.2 (111.8) | 350.66 | 4.19E-06 | 217.50 | 7.92E-06 |
| IL-6 | 17.3 (5.4) | 3,097.2 (1,279.4) | 23.3 (9.4) | 5,458.6 (1,907.6) | 178.52 | 3.58E-06 | 234.66 | 5.53E-06 |
| GRO-α (CXCL1) | 95.6 (46.4) | 1,842.4 (369.4) | 183.9 (75.3) | 2,364.5 (446.6) | 19.28 | 2.06E-05 | 12.86 | 5.01E-05 |
| LIF | 3.2 (0.1) | 60.6 (18.3) | 3.1 (0.0) | 92.1 (33.0) | 19.12 | 4.84E-06 | 29.94 | 4.13E-05 |
| IL-1α | 8.1 (1.3) | 137.8 (33.0) | 11.7 (2.1) | 148.9 (46.1) | 16.95 | 4.75E-05 | 12.77 | 7.83E-05 |
| IL-22 | 11.3 (0.4) | 183.4 (79.0) | 10.9 (0.0) | 159.1 (54.5) | 16.22 | 5.35E-06 | 14.61 | 5.45E-04 |
| G-CSF | 8.5 (3.6) | 127.1 (16.4) | 23.1 (9.7) | 127.7 (17.5) | 14.96 | 7.44E-06 | 5.53 | 8.85E-05 |
| MCP-3 (CCL7) | 19.8 (3.2) | 291.3 (58.8) | 40.2 (6.8) | 256.5 (44.0) | 14.74 | 4.19E-06 | 6.38 | 5.81E-06 |
| IL-1β | 8.6 (1.0) | 118.0 (34.8) | 9.9 (1.5) | 113.6 (34.0) | 13.79 | 3.42E-05 | 11.43 | 0.001 |
| IL-17A (CTLA-8) | 1.6 (0.0) | 10.4 (3.5) | 1.6 (0.0) | 11.0 (3.5) | 6.57 | 1.26E-04 | 7.00 | 1.60E-04 |
| MIP-1α (CCL3) | 36.6 (6.8) | 203.2 (37.5) | 19.3 (2.7) | 188.7 (38.4) | 5.55 | 0.002 | 9.80 | 0.009 |
| IL-27 | 2.1 (0.0) | 11.0 (2.3) | 2.0 (0.0) | 10.9 (2.6) | 5.29 | 1.47E-04 | 5.34 | 1.60E-04 |
| GM-CSF | 3.2 (0.0) | 14.4 (2.8) | 3.1 (0.0) | 13.4 (3.9) | 4.55 | 0.002 | 4.27 | 0.002 |
| TNF-α | 2.8 (0.0) | 12.1 (3.3) | 2.8 (0.0) | 14.5 (4.2) | 4.35 | 0.011 | 5.21 | 0.006 |
| IP-10 (CXCL10) | 59.8 (6.5) | 240.1 (27.1) | 38.9 (5.9) | 204.4 (25.4) | 4.01 | 3.42E-05 | 5.25 | 2.08E-04 |
| Eotaxin | 135.7 (7.4) | 433.9 (55.9) | 150.7 (13.8) | 363.0 (50.1) | 3.20 | 3.56E-05 | 2.41 | 6.45E-04 |
| IL-4 | 2.1 (0.2) | 5.6 (1.9) | 1.4 (0.1) | 5.5 (1.4) | 2.68 | 0.260 | 3.83 | 0.047 |
| IL-13 | 3.1 (0.0) | 6.9 (1.1) | 3.1 (0.0) | 5.6 (0.8) | 2.25 | 0.002 | 1.83 | 0.027 |
| MIP-1β (CCL4) | 103.0 (17.4) | 219.2 (52.0) | 63.0 (7.6) | 189.7 (48.4) | 2.13 | 0.188 | 3.01 | 0.192 |
| IL-12p70 | 2.0 (0.1) | 4.0 (0.7) | 1.9 (0.0) | 3.6 (0.5) | 1.99 | 0.012 | 1.87 | 0.008 |
| IL-10 | 4.4 (0.4) | 7.7 (0.7) | 3.7 (0.3) | 7.3 (0.7) | 1.76 | 0.005 | 1.99 | 0.002 |
| IL-15 | 2.3 (0.0) | 3.9 (0.4) | 2.3 (0.0) | 3.6 (0.5) | 1.70 | 0.002 | 1.61 | 0.007 |
| IL-2 | 1.6 (0.0) | 2.7 (0.3) | 1.6 (0.0) | 2.2 (0.2) | 1.69 | 0.001 | 1.37 | 0.010 |
| IL-5 | 2.5 (0.0) | 4.0 (0.5) | 2.5 (0.0) | 3.2 (0.3) | 1.59 | 0.004 | 1.26 | 0.059 |
| M-CSF | 3.3 (0.6) | 3.7 (0.8) | 1.4 (0.4) | 2.1 (0.5) | 1.13 | 0.985 | 1.46 | 0.915 |
| ENA-78 (CXCL5) | 80.4 (9.0) | 77.9 (8.0) | 65.7 (9.3) | 105.2 (7.8) | 0.97 | 0.804 | 1.60 | 0.005 |
| RANTES (CCL5) | 533.5 (14.6) | 442.3 (17.0) | 437.3 (30.0) | 427.0 (12.5) | 0.83 | 2.65E-04 | 0.98 | 0.149 |
| sample size | 11 | 22 | 11 | 25 |
|
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*All values shown as mean (SEM).
**Mann-Whitney test for comparison of high-burden to low-burden samples within the same sex. Bolded, p < 0.05.