Table 5.
Serum antibody titers against Y. pestis rV antigen in mice vaccinated with Δcaf1 or ΔyopD/Δcaf1 regimens.
| Sex | Vaccine Prime |
Vaccine Boost |
21-day Survival |
n = | Anti-rV Antibody Titer a | |||
|---|---|---|---|---|---|---|---|---|
| Isotype | Ratio IgG2a/IgG1 |
|||||||
| IgG | IgG1 | IgG2a | ||||||
| Female | PBS | PBS | 0% | 4 | 84 (1.60) | 178 (2.21) | 50 (1.00) | n/a |
| Male | PBS | PBS | 0% | 4 | 59 (1.17) | 50 (1.00) | 50 (1.00) | n/a |
| Female | rF1-V | Δcaf1 | 100% | 4 | 574,701 (1.28) | 4,872,974 (1.30) | 48,326 (1.26) | 0.010 |
| Male | rF1-V | Δcaf1 | 50% | 4 | 80,635 (1.53) | 430,539 (1.64) | 2,016 (1.78) | 0.005 |
| Female | Δcaf1 | rF1-V | 10% | 3 | 322,540 (2.39) | 3,002,095 (1.94) | 5,486 (3.59) | 0.002 |
| Male | Δcaf1 | rF1-V | 0% | 4 | 161,270 (1.25) | 542,445 (1.32) | 3,200 (1.34) | 0.006 |
| Female | PBS | PBS | 0% | 4 | 50 (1.00) | 50 (1.00) | 50 (1.00) | n/a |
| Male | PBS | PBS | 0% | 4 | 50 (1.00) | 50 (1.00) | 50 (1.00) | n/a |
| Female | rF1-V | ΔyopD/Δcaf1 | 100% | 4 | 683,605 (2.01) | 2,581,158 (1.36) | 12,800 (3.20) | 0.005 |
| Male | rF1-V | ΔyopD/Δcaf1 | 90% | 4 | 514,028 (1.43) | 2,169,780 (1.54) | 38,205 (2.72) | 0.018 |
| Female | ΔyopD/Δcaf1 | rF1-V | 20% | 4 | 53,817 (1.48) | 341,719 (1.37) | 100 (1.44) | 0.0003 |
| Male | ΔyopD/Δcaf1 | rF1-V | 0% | 4 | 144,815 (1.76) | 512,000 (1.67) | 1,068 (3.10) | 0.002 |
a
Values represent geometric mean with geometric standard error in parentheses.
Bolded, p < 0.05 in Mann-Whitney comparison of males to females with same regimen. Italicized, p < 0.05 in Mann-Whitney comparison of (rF1-V prime: LAV boost) to (LAV prime: rF1-V boost) among same sex.