Table 1.

Primer pairs used for qualitative and quantitative detection of Fusarium pseudograminearum, F. graminearum, F. asiaticum, and Triticum sp. DNA in this study.

Primer	Sequence (5′→3′)	Target	Fragment	Reference
TEF1α.2F	ATCATTCGAATCGCTCGACG
TEF1α.2R	AAAAATTACGACAAAGCCGTAAAAA	F. pseudograminearum	82 bp	Knight et al., 2012
Hor1 F	TCTCTGGGTTTGAGGGTGAC
Hor2 R	GGCCCTTGTACCAGTCAAGGT	Wheat	63 bp	Nicolaisen et al., 2009
Fp1-1	CGGGGTAGTTTCACATTTCCG
Fp1-2	GAGAATGTGATGACGACAATA	F. pseudograminearum	523 bp	Aoki and O’Donnell, 1999
FaF	CAGCTTCCTCGAAGACCT
FaR	GGACCGTAAATTTCTTCAGTG	F. asiaticum	292 bp	Yin et al., 2009
FgF	TATCCCTTATGGGTCTTGGT
FgR	GGACCGTAAACTTCTTCTGCA	F. graminearum	352 bp	Yin et al., 2009