Table 2.
The parameters of quantitative analysis using in an Ultimate 3000 ultrahigh-performance liquid chromatography system coupled with a Q Exactive-Orbitrap High Resolution Mass Spectrometer (LC–MS/MS analysis) for DON, ZEN, NIV, 15ADON, 3ADON, and D3G contents.
| Mycotoxinsx | Molecular ion | Precursor ion (m/z) | RTz (min) | Ion pairy (m/z) | Collision energy (eV) |
|---|---|---|---|---|---|
| DON | [DON-H]- | 295.1171 | 3.19 | 265.1070*/247.0961 | 15 |
| ZEN | [ZEN-H]- | 317.1380 | 7.90 | 175.0380*/273.1481 | 45 |
| NIV | [NIV+CH3COO]- | 371.1331 | 2.13 | 281.1019*/311.1125 | 15 |
| 15ADON | [15ADON+CH3COO]- | 397.1485 | 5.88 | 337.1280*/150.0323 | 10 |
| 3ADON | [3ADON-H]- | 337.1279 | 5.87 | 307.1170*/173.0590 | 15 |
| D3G | [D3G+CH3COO]- | 517.1895 | 3.01 | 427.1582*/457.1689 | 25 |
xDON, deoxynivalenol, its derivatives (D3G, DON-3-glucoside; 3ADON, 3-acetyldeoxynivalenol; 15ADON, 15-acetyldeoxynivalenol; and NIV, nivalenol); and ZEN, zearalenone. y *Quantitative ion. The detection limits of toxins in wheat were 10 µg/kg for DON and NIV, 10 µg/kg for 3ADON, 3 µg/kg for 15ADON, and 1 µg/kg for D3G, respectively. zRT = the retention time of chromatographic peaks.