FIG. 2.

Intracellular forms of NOTCH1 activate the major latent EBV promoters in BJAB cells. The CAT gene was used as the reporter for activity on the Cp1 (A) and −512/+40 LMP1 (B) promoter regions. Ten micrograms of each reporter construct and 20 μg (each) of the constructs cloned into pCDNA3.1 were transfected into BJAB cells to measure the level of transactivation of each reporter construct. Levels labelled LMP1 and Cp1 indicate transfection with reporter alone for basal activity. PCDNA3.1 vector DNA was used to normalize the amount of DNA in each transfection, and 2.5 μg of an expression plasmid containing the β-galactosidase gene was used as an internal control for transfection. Activity was counted on a Molecular Dynamics PhosphorImager and values are expressed as arbitrary units.