FIG. 3.

Intracellular nucleus-localized forms of NOTCH1 repress the major latent EBV promoter elements in the Jurkat cells. Transient CAT reporter assays with the EBV Cp1 promoter (A) and transient transactivation activity on the EBV −512/+40 LMP1 promoter (B) are shown. Cells were transfected with equivalent amounts of DNA, including 10 μg of each reporter plasmid and 20 μg (each) of the various NOTCH1 constructs. The total amount of DNA was normalized by adding vector DNA. Levels labelled LMP1 and Cp1 represent transfection with reporter alone to determine basal levels of activation. The transfected cells were harvested after 22 h and CAT assays were done as described previously (3, 22). Data were collected on a Molecular Dynamics PhosphorImager and values are expressed as arbitrary units.