TABLE 1.
Summary of the DNA binding activities of 35 point mutant proteinsa
| Residue mutated | DNA binding activity |
|---|---|
| 334T | <0.06 |
| 347K | <0.06 |
| 348N | 0.5 |
| 349H | <0.06 |
| 350R | 0 |
| 352R | 0.5 |
| 355N | No expression |
| 365D | 0.25 |
| 366N | wt |
| 367G | wt |
| 369E | wt |
| 371N | 0.5 |
| 372G | <0.06 |
| 373Q | wt |
| 381G | wt |
| 383P | wt |
| 384S | wt |
| 385Q | wt |
| 386R | <0.06 |
| 387Q | wt |
| 388D | wt |
| 389Fb | No expression |
| 390Lb | wt |
| 391K | wt |
| 392H | 0.25 |
| 394P | wt |
| 395Lb | <0.06 |
| 398G | wt |
| 400N | 0.5 |
| 401Ib | wt |
| 402S | wt |
| 403G | <0.06 |
| 405F | 0.03 |
| 409D | 0.03 |
| 410Fb | wt |
a
By using mutant protein concentrations normalized with respect to the wt, the DNA binding activities of all of the point mutant proteins were determined by EMSA using a probe containing a high-affinity E2 binding site. The binding activity of each mutant protein is expressed relative to the activity of the wt E2 DBD.
b
Not on the surface.