Efficiency of patient-derived ToC generation
(A) Workflow from surgery to ToCs and live-imaging microscopy. Right after the surgery, the tumor sample is transferred from the hospital to the research facility. Autologous cancer cells and tumor-infiltrating CD8+ CTLs are isolated by MACS and immediately used to generate ToC platforms.
(B) Efficiency of patient-derived cell isolation. For each patient, the tumor sample size, the number and viability of CD8+ CTLs and cancer living cells retrieved, the number of generated ToC, and the drug treatment are indicated.