Figure 7.

Quantification of CTL-mediated anti-tumor cytotoxic activity in autologous patient-derived lung ToCs

(A) Apoptotic death induced by autologous CD8⁺ CTLs as function of CTL density. ToC data shown correspond to patient #1 using primary cancer cells alone or co-cultured with autologous CD8⁺ TILs at a 1:1 or 1:5 ratio. The apoptosis rates of cancer cells were computed over 24 h using the TM-STAMP method and averaged. The graph reports mean ± SEM from 4 view fields.

(B) Cancer cell survival curves for patient #1. The percentage of surviving cancer cells, calculated with respect to the initial number of living cells, was computed using the TM-STAMP method. The anti-PD-1 drug was added at the beginning of the on-chip co-culture (t0). The graph reports means ± SEM from 4 view fields.

(C) Cancer cell survival curves for patient #2 (1:3 cancer to immune cell ratio).

(D) Cancer cell survival curves for patient #3.

(E) Cancer cell survival curves for patient #7.

(F) Cancer cell survival curves for patient #8.

(G) Cancer cell survival curves for patient #9.

(H) Summary of observed responses in all patients.