Figure 2.

Neutralization effect of anti-gp42 antibodies in B and epithelial cells

(A) The neutralization effect of 2B7, 2C1, 5E3, AMMO1 (anti-gH/gL), 1D8 (anti-gH/gL), 72A1 (anti-gp350), and control IgG was assessed against EBV infection in Raji B cells (n = 3).

(B) qPCR analysis of human leukocyte antigen class II (HLA-II) gene expression in various epithelial cell lines. The expression levels are presented as relative fold change to the GAPDH gene (n = 4).

(C) CNE2-EBV infection rate in different epithelial cell lines. CNE2-EBV was added to each cell in equal amounts (n = 4).

(D) qPCR analysis of HLA-II gene expression in HEK293 cells and HEK293 cells overexpressing HLA-II (HEK293-HLA-II). The expression levels are presented as relative fold change to the GAPDH gene (n = 4).

(E) CNE2-EBV infection rate in HEK293 cells and HEK293-HLA-II. CNE2-EBV was added to each cell in equal amounts (n = 3).

(F and G) The neutralizing activities of mAbs against CNE2-EBV infection in HEK293 cells (F) or HEK293-HLA-II cells (G) (n = 3).

(H) The IC50 values of mAbs in different B and epithelial cell lines (n = 3).

Significance was calculated using one-way ANOVA followed by Tukey’s multiple comparisons (C) and two-tailed unpaired Student’s t test (E). Error bars represent ±SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

See also Figure S2.