TABLE 1.
Clone | Optical density value for SSPE sections
|
|||||
---|---|---|---|---|---|---|
Acid eluted
|
Untreated
|
|||||
Infected | Uninfected | Background | Infected | Uninfected | Background | |
1 | 0.078 | 0.078 | 0.069 | 0.063 | 0.071 | 0.064 |
2 | 0.068 | 0.071 | 0.062 | 0.080 | 0.073 | 0.065 |
3 | 2.188 | 0.092 | 0.081 | 3.189 | 0.082 | 0.071 |
4 | 0.077 | 0.087 | 0.066 | 0.071 | 0.091 | 0.071 |
5 | 0.076 | 0.085 | 0.071 | 0.084 | 0.099 | 0.071 |
6 | 0.086 | 0.079 | 0.081 | 0.101 | 0.119 | 0.127 |
7 | 0.079 | 0.091 | 0.075 | 0.087 | 0.103 | 0.116 |
8 | 0.071 | 0.072 | 0.066 | 0.089 | 0.118 | 0.116 |
9 | 0.092 | 0.097 | 0.080 | 0.070 | 0.106 | 0.114 |
10 | 2.139 | 0.105 | 0.086 | 0.074 | 0.109 | 0.106 |
11 | 0.076 | 0.082 | 0.070 | 0.064 | 0.071 | 0.064 |
12 | 0.069 | 0.087 | 0.066 | 0.069 | 0.080 | 0.066 |
13 | 0.082 | 0.089 | 0.067 | 0.066 | 0.076 | 0.063 |
14 | 0.196 | 0.192 | 0.150 | 0.086 | 0.084 | 0.063 |
15 | 0.081 | 0.088 | 0.072 | 0.107 | 0.132 | 0.127 |
16 | 0.065 | 0.068 | 0.064 | 0.139 | 0.154 | 0.145 |
17 | 0.074 | 0.086 | 0.075 | 0.144 | 0.170 | 0.137 |
18 | 0.194 | 0.207 | 0.110 | 0.161 | 0.149 | 0.075 |
19 | 0.434 | 0.080 | 0.074 | 0.197 | 0.181 | 0.090 |
20 | 0.127 | 0.115 | 0.083 | 0.136 | 0.090 | 0.069 |
IF9b | 1.301 | 0.076 | 0.062 | 1.381 | 0.085 | 0.062 |
ELISA measures binding of soluble Fabs to detergent extracts of MV-infected and uninfected Vero cells. Background binding measures immunoreactivity of secondary antibody to MV-infected cell lysates in the absence of soluble Fab. Optical density was measured at 405 nm after a 60-min incubation with p-nitrophenol phosphate as substrate.
IF9 is an MV phosphoprotein-specific Fab obtained by panning on MV-infected cell lysates (6).