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. 2024 Jun 4;24:197. doi: 10.1186/s12935-024-03377-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — STX6 promotes migration and invasion of HCC cells. (A) The representative images and quantification results of Transwell migration assay of Huh7/HepG2 STX6-OE and corresponding control cells. n = 3 per group. (B) The representative images and quantification results of Huh7 STX6-OE and corresponding control cells. n = 3 per group in wound healing assay. (C) The representative images and quantification results of Huh7/HepG2-STX6-OE and corresponding control cells in Transwell invasion assay. n = 3 per group. (D-E) Protein levels and quantification results of metastasis-related E-cadherin and N-cadherin in Huh7/HepG2 STX6-OE and corresponding control cells. n = 3 per group. (F) The representative images and quantification results of Huh7/HepG2 shSTX6 and corresponding control cells in Transwell migration assay. n = 3 per group. (G) The representative images and quantification results of wound healing assay of Huh7 shSTX6 and corresponding control cells. n = 3 per group. (H) The representative images and quantification results of Transwell invasion assay of Huh7/HepG2 shSTX6 and corresponding control cells. n = 3 per group. (I-J) Protein levels and quantification results of metastasis-related E-cadherin and N-cadherin in Huh7/HepG2 shSTX6 and corresponding control cells. n = 3 per group. β-actin served as the loading control. Data was given as mean ± SD. For statistical analysis, the two-tailed Student’s t-test was used in A-C, E-H, and J. * P < 0.05; ** P < 0.01