| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-HSP47 | proteintech | Cat# 10875-1-AP; RRID: AB_2301500 |
| Anti-HSP47 | Santa Cruz | Cat# sc-5293; RRID: AB_627757 |
| Anti-N-cadherin | proteintech | Cat# 22018-1-AP; RRID: AB_2813891 |
| Anti-E-cadherin | proteintech | Cat# 20874-1-AP; RRID: AB_10697811 |
| Anti-Snail | proteintech | Cat# 13099-1-AP; RRID: AB_2191756 |
| Anti-Collagen I | proteintech | Cat# 14695-1-AP; RRID: AB_2082037 |
| Anti-Collagen I | proteintech | Cat# 67288-1-Ig; RRID: AB_2882554 |
| Anti-Collagen II | proteintech | Cat# 28459-1-AP; RRID: AB_2881147 |
| Anti-Collagen III | proteintech | Cat# 22734-1-AP; RRID: AB_2879158 |
| Anti-Collagen IV | proteintech | Cat# 19674-1-AP; RRID: AB_2878595 |
| Anti-GFP-Tag | Abclonal | Cat# AE012; RRID: AB_2770402 |
| Anti-Arg1 | proteintech | Cat# 16001-1-AP; RRID: AB_2289842 |
| Anti-CD163 | proteintech | Cat# 16646-1-AP; RRID: AB_2756528 |
| Anti-CD206 | proteintech | Cat# 18704-1-AP; RRID: AB_10597232 |
| Anti- CD11c | proteintech | Cat# 17342-1-AP; RRID: AB_2129787 |
| Anti-IBA1 | proteintech | Cat# 10904-1-AP; RRID: AB_2224377 |
| Anti-CD8 | Abcam | Cat# ab217344; RRID: AB_2890649 |
| Anti-CD8 | Santa Cruz | Cat# sc-7970; RRID: AB_627208 |
| Anti-P65 | CST | Cat# 8242; RRID: AB_10859369 |
| Anti-Phospho-P65 | CST | Cat# 3033; RRID: AB_331284 |
| Anti-Tubulin | Ray antibody | Cat# RM2007; RRID: AB_2934267 |
| CD11b-BV605 | Biolegend | Cat# 101257; RRID: AB_2565431 |
| CD11c-APC | Biolegend | Cat# 117310; RRID: AB_313779 |
| CD206-PE | Biolegend | Cat# 141706; RRID: AB_10895754 |
| CD45-PE/cy7 | Biolegend | Cat# 103114; RRID: AB_312979 |
| CD45-APC/cy7 | BD Pharmingen | Cat# 557659; RRID: AB_396774 |
| CD3-PerCP/cy5.5 | Biolegend | Cat# 100218; RRID: AB_1595492 |
| CD3-FITC | Biolegend | Cat# 100204; RRID: AB_312661 |
| CD4-PE | Biolegend | Cat# 100408; RRID: AB_312693 |
| CD8-APC/Fire™ 750 | Biolegend | Cat# 100766; RRID: AB_2572113 |
| CD8− PerCP/cy5.5 | BD Pharmingen | Cat# 551162; RRID: AB_394081 |
| GZMB-BV421 | Biolegend | Cat# 396414; RRID: AB_2810603 |
| TNF-α-BV650 | Biolegend | Cat# 506333; RRID: AB_2562450 |
| IFN-γ-BV510 | Biolegend | Cat# 505842; RRID: AB_2734494 |
| Ki67-BV786 | BD Pharmingen | Cat# 563756; RRID: AB_2732007 |
| CD69-AF700 | Biolegend | Cat# 104539; RRID: AB_2566304 |
| CD25-BV605 | BD Pharmingen | Cat# 563061; RRID: AB_2737982 |
| FOXP3-AF647 | BD Pharmingen | Cat# 560401; RRID: AB_1645201 |
| Bacterial and virus strains | ||
| EF1A-MCS-SV40-firefly_Luciferase-IRES-Puromycin | Genechem | https://www.genechem.com.cn/ |
| pCLenti-EF1-Neo-CMV-HSP47-WPRE | HanBio | https://hanbio.net/ |
| Tet-pLKO-neo | YouBio | http://www.youbio.cn/vector |
| CMV-MCS-3flag-EF1-GFP-T2A-Blasticidin | Genechem | https://www.genechem.com.cn/ |
| Biological samples | ||
| Human primary breast cancer tissues and paired brain metastatic tissues | Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) | N/A |
| Human primary lung cancer tissues and paired brain metastatic tissues | Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Puromycin | Thermo Fisher Scientific | Cat# A1113803 |
| Neomycin | Solarbio | Cat# IN0130 |
| Blasticidin | Selleck | Cat# S7419 |
| Col003 | MedChemExpress | Cat# HY-124817 |
| TC-I 15 | MedChemExpress | Cat# HY-107588 |
| InVivoMAb anti-mouse CD8a | Selleck | Cat# A2102 |
| IgG2b isotype control-InVivo | Selleck | Cat# A2116 |
| InVivoMAb anti-mouse PD-L1 (B7-H1) | Bio X Cell | Cat# BE0361; RRID: AB_2927503 |
| PLX3397 | MolDiets | Cat# M18072603 |
| Cultrex rCollagen I | R&D Systems | Cat# 3440-100-01 |
| Collagenase 1 | Worthington | Cat# LS004197 |
| ACK Lysis Buffer | LEAGENE | Cat# CS0001 |
| Dnase 1 | Solarbio | Cat# D8071 |
| DAPI | Abcam | Cat# ab104139 |
| Citrate Sodium Buffer, pH 6.0 | Solarbio | Cat# C1010 |
| Tris-EDTA buffer, pH 9.0 | Elabscience | Cat# E-IR-R104 |
| Fetal Bovine Serum | Gibco | Cat# 10099141 |
| Tween 20 | Beyotime | Cat# ST1727 |
| Triton X-100 | Beyotime | Cat# ST797 |
| RIPA buffer | Beyotime | Cat# P0013B |
| Trizol reagent | Invitrogen | Cat# 15596026 |
| D-Luciferin potassium salt | Beyotime | Cat# ST196 |
| Lipofectamine 2000 reagent | Invitrogen | Cat# 11668019 |
| Critical commercial assays | ||
| RNeasy Mini Kit | Qiagen | Cat# 74104 |
| PrimeScript™ RT reagent Kit | TAKARA | Cat# RR037A |
| CUT&Tag Kit | YEASEN | Cat# 12598ES12 |
| True-Nuclear Transcription Factor Buffer Set | Biolegend | Cat# 424401 |
| Mouse Collagen Type I ELISA Kit | CUSABIO | Cat# CSB-E08083m |
| Mouse PBMC Isolation Kit | IPHASE | Cat# 071E100.11 |
| Mouse CD8+ T cell Isolation Kit | IPHASE | Cat# 071E203.11 |
| Deposited data | ||
| RNA-seq of BV2 cells | This paper | GEO: GSE261941 |
| Experimental models: Cell lines | ||
| 4T1 | Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) | N/A |
| LLC1 | Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) | N/A |
| BV2 | BeNa Culture Collection (Beijing, China) | N/A |
| 293FT | BeNa Culture Collection (Beijing, China) | N/A |
| Experimental models: Organisms/strains | ||
| BALB/C mice | Guangdong Medical Laboratory Animal Center (Foshan, China) | N/A |
| C57BL/6 mice | Guangdong Medical Laboratory Animal Center (Foshan, China) | N/A |
| Oligonucleotides | ||
| Primer sequences are provided in the Table S1 | This paper | N/A |
| Software and algorithms | ||
| FlowJo | BD Biosciences | https://www.flowjo.com/ |
| Prism, v8 | GraphPad | https://www.graphpad.com/scientificsoftware/prism/ |
| ImageJ | NIH Image | https://imagej.nih.gov/ij/index.html |
| R (version 4.3.2) | The R Project for Statistical Computing | https://www.r-project.org/ |
| R Studio | PBC | https://www.rstudio.com/ |
| ComplexHeatmap (R package, version 2.16.0) | N/A | https://bioconductor.org/packages/release/bioc/html/ComplexHeatmap.html |
| circlize (R package, version 0.4.15) | N/A | https://jokergoo.github.io/circlize_book/book/ |
| tidyverse (R package, version 2.0.0) | N/A | https://www.tidyverse.org/learn/ |
| ggplot2 (R package, version 3.4.4) | N/A | https://ggplot2.tidyverse.org |
| UMAP | N/A | https://arxiv.org/abs/1802.03426 |
| ggvenn (R package, version 0.1.10) | N/A | https://CRAN.R-project.org/package=ggvenn |
| AnnotationDbi (R package, version 1.62.2) | Bioconductor | https://bioconductor.org/packages/AnnotationDbi |
| clusterProfiler (R package, version 4.8.3) | Wu et al.39 | https://yulab-smu.top/biomedical-knowledge-mining-book/ |
| enrichplot (R package, version 1.20.3) | Bioconductor | https://bioconductor.org/packages/release/bioc/html/enrichplot.html |
| FastQC v0.11.9 | N/A | https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ |
| Trim galore v0.6.7 | N/A | https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/ |
| Hisat2 v2.2.1 | Kim et al.41 | http://daehwankimlab.github.io/hisat2/ |
| DEseq2 (R package, version 1.40.2) | Love et al.42 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| infercnvpy v0.4.3 | N/A | https://infercnvpy.readthedocs.io/en/latest/ |
| Seurat (R package, version 5.0.1) | N/A | http://satijalab.org/seurat |
| scanpy v1.9.3 | N/A | https://scanpy.readthedocs.io/en/stable/ |
| Other | ||
| PVDF membrane | Millipore | Cat# IPVH00010 |
| 24-well plate | Corning | Cat# 3524 |
| 0.45 μm filter | Sorfa | Cat# 622120 |
| 70 μm cell filter | ABCbio | Cat# ABC60070 |
| Transwell insert | Corning | Cat# 3422 |
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