FIG. 1.
Analysis of GPCMV concatemer termini. (A) The two GPCMV genome structures are illustrated with the terminal repeats (shown as hatched boxes) and the sizes (in kilobases) of terminal HindIII fragments indicated. Thin lines indicate the locations of pac1, pac2M, and the cryptic element pac2O. Thick lines indicate regions used as hybridization probes. (B) Autoradiograph of DNA blot hybridizations to detect GPCMV terminal restriction fragments and concatemer junctions. Concatemeric and 230-kb intracellular DNAs were prepared by FIGE and digested with HindIII, then separated by agarose electrophoresis, transferred to a nylon membrane, and sequentially hybridized with probes A and B.