FIG. 2.

Analysis of MCMV concatemer termini. (A) The MCMV genome is illustrated, showing terminal SalI C and X fragments. Below it a concatemer junction with the CX junction fragment is illustrated. The sizes of the SalI fragments are indicated (in kilobases). Thin lines indicate the locations of pac1 and pac2, and the thick line indicates the region used as a hybridization probe. (B) Autoradiograph of DNA blot hybridization to detect MCMV terminal restriction fragments and concatemer junctions. Concatemeric, 230-kb intracellular, and 230-kb virion DNAs were prepared by FIGE and digested with SalI, then separated by agarose electrophoresis, transferred to a nylon membrane, and hybridized with probe C.