Fig. 1. Screening of chemical compounds using the iPS-derived T cells.

a T cell differentiation scheme from T-iPSCs for screening chemical compounds. Expanded T-iPS-T cells were stimulated with CD3/CD28 microbeads and then incubated with compounds. All data presented in Fig. 1 were acquired 6 d later. b Cell numbers based on intracellular dehydrogenase activity. Error bars indicate the standard deviation (SD) of five independent experiments. c Representative FACS plots 6 d after the addition of the compounds. d Percentage and e number of PI-viable cells determined with flow cytometry. f Percentage and g number of CCR7+CD45RA+ cells. Error bars indicate the SD of four independent experiments (c–f). Statistical significance is denoted as follows: *p < 0.05; **p < 0.01; and ***p < 0.001 (paired t-test).