Fig. 4. Gene expression analysis in BBM-treated stem cell memory T cells.
a Gating strategy for FACS to purify the naïve and memory T cell fractions from healthy donor-derived CD8+ cells. b, c Gene ontology analysis of DEGs in BBM-treated TSCM cells. Upregulated (b) or downregulated (c) genes from RNA-sequencing results on day 12. Genes were queried in the DAVID functional annotation database. d Transcripts per million (TPM) values of cell cycle-related and effector function-related genes. Statistical significance is denoted as follows: *p < 0.05; **p < 0.01; and ***p < 0.001 (n = 4, paired t-test). TSCM stem cell memory T, TCM central memory T, TEM effector memory T.