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. 2024 Jun 4;7:685. doi: 10.1038/s42003-024-06297-0

Fig. 6. Effector functions of second-generation CAR-T cells.

Fig. 6

a CD19-CAR retrovirus vectors (top) and an experimental scheme for in vitro assays. b Assessment of cell proliferation among CD3+ T cells transduced with CD19-CAR (CAR-T cells). Fold expansion rates compared to Day 0 were shown. c Evaluation of in vitro cytotoxicity of CAR-T cells against CD19-expressing NALM6 cells. E/T ratio effector/target ratio. Error bars indicate SD from three donors (b, c). d Expression of CD107a and e IFNγ production in CAR-T cells that were stimulated with PMA plus Ionomycin (PMA/Ionomycin), NALM6 cells, or unstimulated control (T cell only). Circle; 19-28z, triangle; 19-BBz. Statistical significance is denoted as follows: *p < 0.05; **p < 0.01; and ***p < 0.001 (paired t-test).