Skip to main content
. 2024 Jun 4;15:4575. doi: 10.1038/s41467-024-48579-5

Fig. 10. Vascular changes via Notch or VEGF-A signaling do not affect with calvarial bone regeneration.

Fig. 10

a Whole mount multiphoton microscopy showing Emcn+ (green) microvessels in Dll4 iΔEC and control mice after calvarial bone lesions at PLD14. SHG+ calvarial bone (cb) and new bone (nb) are shown in blue. Note areas of hyperbranching and defects in vascular maturation (arrowheads). b Histograms showing vascular area (top) and newly formed bone are (bottom) in calvarial lesions (as 100%) in control and Dll4 iΔEC mice at PLD14. Data are presented as mean values ± SD. n = 7 (vessel area) and n = 8 (bone area) independent drill holes were examined over 4 biologically independent animals. Unpaired t test, two-tailed was used for control vs. EC-specific Dll4 mutants. Vessel area p < 0.0001. Bone area p = 0.9494. c Whole mount multiphoton microscopy showing Flk1-GFP+ (green) microvessels in mice injected with pLIVE Vegfa and pLIVE control three days prior to calvarial bone lesion injury, and analyzed at PLD14. SHG+ calvarial bone (cb) and new bone (nb) are shown in blue. Note microvessels with partially compromised vascular lumen (arrow heads). d Histograms showing vascular area (top) and area of newly formed bone (bottom) in calvarial lesions (as 100%) in Flk1-GFP+ mice injected with pLIVE Vegfa and pLIVE control at PLD14. Data are presented as mean values ± SD. n = 6 (vessel area) and n = 7 (bone area) independent drill holes were examined over 4 biologically independent animals. Unpaired t test, two-tailed was used for pLIVE control vs. pLIVE Vegfa. Vessel area p = 0.0001. Bone area p = 0.6557. Source data are provided as a Source Data file.