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. 2024 Jun 5;14:12874. doi: 10.1038/s41598-024-62828-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Skin histological improvement by ESS treatment in DNCB-induced BALB/c mice. In DNCB-induced BALB/c mice, we observed histopathological features of the skin that were altered by ESS treatment. Changes in epidermal thickness of the back skin induced by atopic dermatitis-like symptoms were observed with (a, c) H&E staining (n = 12 pictures per group), and changes in dermal infiltration of mast cells were observed with (b, d) Toluidine Blue staining (n = 20 pictures per group). The values presented are the mean ± SEM from independent experiments. Statistical significance is indicated as follows: ***p < 0.001, **p < 0.01, and *p < 0.05. Scale bar = 100 μm.