Figure 3.
Detection of model pathogen S. pneumoniae. (A) Slide arrays processed in open well format. (B, C) Measurement of increasing numbers of bacteria, captured by different amounts of antibody in individual spots. Analyte was applied either in PBST (B) or in saliva (C) (each N = 3). Graphs in the right panel depict results of linear regression analysis assessing the relationship between fluorescence read-out (relative fluorescence unists, RFU) and analyte concentration in the samples. Note that the highest concentration of bacteria (1 × 108 CFU/ml) has been omitted from all regression analyses.