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. 2024 Jun 4;14:12830. doi: 10.1038/s41598-024-62784-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of markers on glass slides using a microfluidic device. (A) Slide with four reaction arrays carrying duplicate spots of capture antibodies for the detection of four different analytes. (B) Detection of analytes in individually spiked saliva samples (analyte concentration: 10 ng/ml) using a mixture of biotinylated detection antibodies. (C) Detection of analytes in mixed spiked saliva samples (IL-6 & IL-8: 10 ng/ml, TNF-α: 25 ng/ml, S. pneumoniae: 2 × 10⁵ CFU/ml) using individual biotinylated detection antibodies in each reaction chamber.