Figure 4.

Cn tps1Δ generates an initial rapid inflammation in the lungs that quickly resolves the infection. BALB/c mice were infected IT with 1*10⁷ cells of Cn H99, tps1Δ, or tps1Δ:TPS1. H&E-stained histological images of mouse lung at 1 dpi and 3 dpi (10× objective). Note that mice infected with either strain induced similar levels of lung inflammation, but by 3 dpi, tps1Δ-infected lungs have mostly contained the infection with minimal inflammation or damage remaining; in contrast, the H99 and tps1Δ:TPS1-infected lungs show progressive infection with increasing presence of cryptococcal cells (indicated with yellow arrows), and severe pathology.