Figure 6.
The Cn tps1Δ remains avirulent in the lungs, even at the extremely high inoculum. BALB/c mice were infected IT with 1*107 cells of Cn H99, tps1Δ, or tps1Δ:TPS1. Fungal lung burdens (A) at corresponding timepoints (three experiments; n = 7–11 mice/group) and the survival analysis (B) (n = 4–5 mice/group) support the histological findings. (C) Lung fungal burdens evaluated for H99 at the time of death (5 dpi–6 dpi) show its progressive expansion. Lung fungal burdens of tps1Δ-infected lungs were evaluated at 2 weeks postinfection, showing fungal containment and 3 weeks postinfection, showing fungal clearance from the lung; n = 3–4 mice/group. *p ≤ 0.05.