Figure 7.

Cryptococcal tps1-gene deletion renders Cn susceptible to the acute resident pulmonary defenses but not the antimicrobial serum effects. (A) Dispersed lung cells enriched in leukocytes were isolated from naïve BALB/c mice and cocultured with either H99- or tps1Δ Cn for 4 h at 37°C and plated for surviving fungal numbers. Data shown (means ± SEM) are the cumulative results of two independent experiments (n = 8) and (B) H99 or tps1Δ Cn was incubated with serum for 0 h–4 h with viability assessed every 2 (h) No differences were seen in survival in any of the strains. ****p ≤ 0.0001.