FIG. 8.
Autoradiographic image of histone H1 phosphorylated in vitro by immune precipitates of cyclin B-cdc2 complex from replicate cultures of HeLa cells mock infected or infected with HSV-1(F), R7356 (UL13−), or R7802 (α22−/US1.5−). The cyclin B-cdc2 complex was precipitated with cyclin B antibody from lysates of cells harvested 16 h after infection. The reaction mixtures consisted of the precipitate alone or precipitate plus histone H1. The mixtures were denatured and separated on denaturing polyacrylamide gels at the completion of the reaction and prior to autoradiography. The procedures were as described in the legend to Fig. 1 and in Materials and Methods.