Figure 4.

Transcriptome sequencing analysis results. Control refers to the standard group, the Model represents the epilepsy model group, and KD stands for the group on a classic ketogenic diet. Each group had a sample size of n = 3. (A) Comparison of the Model group with the Control group, red dots indicate upregulated gene expression, green dots indicate downregulated gene expression, the p-value represents the significance of differential gene expression, and a higher Log2FC (log2 fold change) indicates a more difference in expression. (B) Comparison of the epilepsy model group with the classic KD group, p < 0.05, indicating a difference. Red dots indicate upregulated gene expression, green dots indicate downregulated gene expression, the p-value represents the significance of differential gene expression, and a higher Log2FC (log2 fold change) indicates more expression differences. (C) Hierarchical clustering analysis of differentially expressed genes between the Control and Model groups. (D) Hierarchical clustering analysis of differentially expressed genes between the Model and KD groups. (E) Venn diagrams depicting the intersection of upregulated genes in the Model group (compared to the Control group) with downregulated genes in the KD group (upper diagram), and the intersection of downregulated genes in the Model group (compared to the Control group) with upregulated genes in the KD group (lower diagram). Green represents genes differentially expressed between the Control and Model groups, blue represents genes differentially expressed between the Model and KD groups, and the overlapping area indicates intersecting genes. (F) Protein interaction analysis among the Control, Model, and KD groups. (G) GO enrichment analysis. (H) KEGG pathway enrichment analysis.