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Detection of Gag VLPs. Gag VLPs were purified from culture media of Sf9 cells expressing Gag with the C-terminal truncations described and subjected to Western blotting using anti-HIV-1 CA (A) and antipolyhistidine (B) monoclonal antibodies. Lanes: M, prestained molecular weight markers (Bio-Rad); 1 to 5, Gag VLP fractions purified from the supernatant of Sf9 cells expressing MA-CA, MA-CA-p2, MA-CA-p2-NCdl1, MA-CA-p2-NCdl2, and MA-CA-p2-NC, respectively.
