TABLE 1.

Titration of HSV propagated on BHK-21 and tsBN67 cells^a

MOI	Time (h) p.i.	Titer of virusb grown on indicated cell line
		33.5°C		39.5°C
		BHK-21	tsBN67	BHK-21	tsBN67
0.1 PFU/cell	24	1.5 × 107	6 × 106	1.7 × 107	2.1 × 105
	48	2.4 × 107	8.4 × 107	4 × 107	3 × 106
10 PFU/cell	14	3.6 × 106	2.4 × 106	6 × 106	1.4 × 105

^aBHK-21 or tsBN67 cells grown for 48 h at the permissive (33.5°C) or nonpermissive (39.5°C) temperature were infected with 0.1 or 10 PFU of HSV-1 per cell. Total progeny was harvested at 14 h p.i. (MOI of 10 PFU/cell) and at 24 and 48 h p.i. (MOI of 0.1 PFU/cell). 

^bDetermined by plaque assay titration on Vero cells of total intracellular plus extracellular virus.