FIG. 3.

Cell surface location of p80. Biotinylated HepG2 cell lysates were divided into three fractions; one was subjected to the pre-S1 binding assay (lane 1), and the other two were incubated with protein A-Sepharose. The precleared lysate was immunoprecipitated with rabbit anti-human Cdc2 antibody (Santa Cruz Biotechnology) and protein A-Sepharose. The precleared supernatant (lane 2 and 4) and immunoprecipitates (lane 3 and 5) were subjected to Western analysis using streptavidin-HRP (lanes 1 to 3) or rabbit anti-human Cdc2 antibody and anti-rabbit IgG-HRP (lanes 4 and 5). The bands corresponding to 50 and 25 kDa are likely the heavy and light chains, respectively, of rabbit anti-human Cdc2 antibody (lane 5). Molecular size markers (M; in kilodaltons) are shown in the middle, and the positions of p80 and Cdc2 are indicated.