FIG. 6.

p80 binding of pre-S1 mutants with internally deleted segments. (A) Amino acid residues 12 to 20 (lane 3), 73 to 90 (lane 5), or both (lane 7) were deleted from GST–pre-S1(1-56) (lane 2), GST–pre-S1(57-119) (lane 4), or GST–pre-S1(1-119) (lane 6), respectively, and these wild-type and mutant proteins as well as GST (lane 1) were expressed and purified. (B) Each protein was analyzed for p80 binding. Molecular size markers (in kilodaltons) are shown in lane M, and the position of p80 is indicated on the right.