FIG. 7.

Effect of pre-S1 peptides on the binding of p80 to pre-S1. Biotinylated HepG2 lysates were incubated with GST (lane 1), and precleared lysates were incubated with GST–pre-S1 fusion protein in the presence of PBS (lane 2), 100 μg of peptide pre-S1(21-47) (lane 3), 100 μg of peptide pre-S1(12-20) (lane 4), 100 μg of peptide pre-S1(73-90) (lane 5), 50 μg of peptide pre-S1(12-20) plus 50 μg of peptide pre-S1(73-90) (lane 6), or 100 μg of peptide pre-S1(12-20) plus 100 μg of peptide pre-S1(73-90) (lane 7), and the binding experiment was done as described for Fig. 1. Molecular size markers (M; in kilodaltons) are shown on the left, and the position of p80 is indicated on the right.