FIG. 9.

p80 binding of pre-S1 of the ayw subtype of HBV. GST (lane 1) and GST fusion proteins with pre-S1(1-45) (lane 2), pre-S1(46-108) (lane 3), or pre-S1(1-108) (lane 4) of the ayw subtype were expressed and purified (A). The biotinylated HepG2 cell lysates were incubated with GST (lane 1), and the precleared lysates were individually incubated with each of the GST–pre-S1 fusions for p80 binding (B). For comparison, GST (lane 1), GST–pre-S1(1-56, adr subtype) (lane 2), and GST–pre-S1(1-45, ayw subtype) (lane 3) were expressed (C), and the binding experiment was carried out (D). Positions of molecular size markers (M; in kilodaltons) and p80 are indicated.