FIG. 1.
Sequence arrangement of plasmids and viruses used in this study. (A) Schematic diagram of the HSV-1 genome in prototype arrangement, showing the unique sequences (lines) flanked by inverted repeats (boxes). (B) Expansion of the region of the HSV genome cloned in pRR1060, showing the positions of viral open reading frames (arrows) and selected restriction enzyme cleavage sites. (C) Schematic diagram of the plasmid pRR1099, produced by ligating the NheI-EcoNI fragment of pRR1060 between the NruI and XbaI sites of pcDNA3. (D) Schematic diagrams of the genomes of HSV-1 recombinants used in this study. The names and properties of these viruses as they relate to TK and UL34 expression are indicated beneath each diagram. Restriction enzyme abbreviations: B, BglII; E, EcoNI; Nh, NheI; S, SpeI.