FIG. 3.
Expression of UL33, UL34, and UL35 mRNAs. Shown are autoradiographic images of Northern blots of formaldehyde agarose gel-separated RNAs purified from infected Vero cells and probed with RNA probe antisense to the UL34 gene. (A) RNAs purified at various times after infection in the presence or absence of PAA. (B) RNAs purified at various times after infection with Δ305 (lanes 2, 5, 8, 11, and 14), the UL34 deletion virus vRR1072 (lanes 3, 6, 9, 12, and 15), or the homologous repair virus vRR1072Rep (lanes 4, 7, 10, 13, and 16), separated on a 1.3% agarose gel and probed with labeled RNA antisense to sequences between the BspEI and XbaI sites that flank the UL34 gene. Arrowheads indicate the migration positions of wild-type UL33 and UL34 mRNAs. These mRNAs migrate more slowly in the deletion virus due to the insertion of GFP sequences. (C) Same as panel B but run on a 1.8% agarose gel and probed with labeled RNA antisense to sequences between the BstBI and BspEI restriction sites that flank the UL35 gene. The region of the gel containing UL35 mRNA is shown, and an arrowhead indicates the migration position of UL35 mRNA.