FIG. 5.
Single-step growth of UL34 deletion, wild-type, and repair viruses on different cell lines. Shown are plots of the logarithm of PFU of virus accumulated in total culture or in medium against time after infection. Cultures were infected, harvested, and titered on 143/1099E cells as described in Materials and Methods. (A) Growth and release of vRR1072 on noncomplementing 143B cells and complementing 143/1099E cells. Each point represents the mean of three independent experiments; error bars indicate the sample standard deviation. (B to D) Growth of Δ305, vRR1072, and vRR1072Rep on HEp-2 (B), HEL299 (C), and Vero (D) cells. Results of representative experiments are shown.