FIG. 3.
To investigate the potential inhibitory role of normal serum proteins, HIV antibodies, and acute-phase proteins in HIV-1 capture, HIV-1Ba-L-MΦ was first incubated in PBS, NHS, HIV antibody-positive serum (HIV+), and serum from a patient with acute hepatitis A infection (HEP.A). Virus was subsequently captured by using anti-CD36 antibody, and the experiment showed that both HIV+ and HEP.A sera were markedly inhibitory to virus capture. However, use of the virus purification algorithm substantially overcame the inhibitory effects of the HIV+ and HEP.A sera on capture. Data are representative of two independent experiments with <15% variability in the magnitude of capture.