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. Author manuscript; available in PMC: 2024 Jun 5.
Published in final edited form as: J Immunol. 2021 Jan 25;206(5):1088–1101. doi: 10.4049/jimmunol.2001097

FIGURE 5.

FIGURE 5.

Ig responses in the skin mucus and serum from infected and surviving fish. (A) Concentration of IgT, IgM, and IgD in skin mucus from control, infected, and survivor fish (n = 12). (B) Western blot analysis of IgT-, IgM- and IgD-specific binding to F. columnare in skin mucus (dilution 1:2) from infected and survivor fish. (C and D) IgT-, IgM- and IgD-specific binding to F. columnare in dilutions of skin mucus from infected (C) and survivor (D) fish evaluated by densitometric analysis of immunoblots and presented as relative values to those of control fish (n = 12–18). (E) Concentration of IgT, IgM, and IgD in serum from control, infected, and survivor fish (n = 12). (F) Western blot analysis of IgT-, IgM- and IgD-specific binding to F. columnare in serum (dilution 1:10) from infected and survivor fish. (G and H) IgT-, IgM- and IgD-specific binding to F. columnare in dilutions of serum from infected (G) and survivor (H) fish evaluated by densitometric analysis of immunoblots and presented as relative values to those of control fish (n = 12–18). Data are representative of at least three independent experiments (mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired Student t test.