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Relative amplification efficiency of influenza virus vRNPs reconstituted from templates of various lengths. Diagram of the structure of the NS segment (NS) and the collection of deletion derivatives, indicating the sequences deleted, the transcript length (in parentheses), and the relative activity in the in vitro transcription assay (taking clone 105 as a reference). The black regions at the ends represent the sequences conserved among all influenza virus RNA segments.
