Author response image 2. Molecular dynamics simulations showing that phosphatidylinositol is not sufficient for STX17TM insertion into membranes.

(E) An example of a time series of simulated results of STX17TM insertion into a membrane consisting of 70% phosphatidylcholine (PC), 20% phosphatidylethanolamine (PE), and 10% phosphatidylinositol (PI). STX17TM is shown in blue. Phosphorus in PC, PE and PI are indicated by yellow, cyan, and orange, respectively. Short-tailed lipids are represented as green sticks. The time evolution series are shown in Video 3. (F) Time evolution of the z-coordinate of the center of mass (z_cm) of the transmembrane helices of STX17TM in the case of membranes with PI. Five independent simulation results are represented by solid lines of different colors. The gray dashed lines indicate the locations of the lipid heads. A scale bar indicates 5 nm.