FIG. 1.
Comparison of SDV growth in RTG2 cells at 10 and 14°C. (Rows A to C) RTG-2 cells were infected with serial dilutions of SDV (1 to 10−5 [columns 1 to 6, respectively]) starting from the inoculum concentration indicated on the left. Plates were incubated at 10°C (upper) or 14°C (lower). At 7 days p.i. SDV-infected cells were overinfected with VHSV, and 3 days later they were overlaid with agarose. SDV-infected cell foci were visualized following crystal violet staining. (Rows D) Uninfected cells (except column 1 at 10°C, where cells were infected only with VHSV).