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Immunodetection of SDV E2 glycoprotein. The partial SDV E2 protein produced in E. coli and concentrated SDV virions were separated on an SDS–12% polyacrylamide gel, transferred onto a polyvinylidene difluoride membrane, and incubated with a rabbit E2 antiserum. Lane 1, recombinant E. coli ΔE2 protein; lane 2, SDV virions. Positions of molecular weight markers (in thousands) are indicated on the left.
