KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-GFP | Abcam | Cat #13970; RRID: AB_300798 |
| Anti-mCherry | Abcam | Cat #ab167453; RRID: AB_2571870 |
| Anti-nc82 | DSHB | Cat #2314866; RRID: AB_2314866 |
| Alexa Flour 488 anti-chicken | Molecular Probes | Cat #A-11039; RRID: AB_142924 |
| Alexa Flour 555 anti-rabbit | AAT Bioquest | Cat #16690 |
| Alexa Flour 647 anti-mouse | AAT Bioquest | Cat #16562 |
| Chemicals, peptides, and recombinant proteins | ||
| Dopamine (DA) | Sigma-Aldrich | Cat #H8502 |
| Acetylcholine (ACh) | Solarbio | Cat #G8320 |
| Mecamylamine (Meca) | Sigma-Aldrich | Cat #M9020 |
| Tiotropium (Tio) | Dexinjia Bio &Tech | N/A |
| All-trans-retinal | Sigma-Aldrich | Cat #R2500 |
| 5-hydroxytryptamine (5-HT) | Tocris | Cat #3547 |
| Deschloroclozapine (DCZ) | MedChemExpress | Cat #HY-42110 |
| Octopamine (OA) | Tocris | Cat #2242 |
| Glutamate (Glu) | Sigma-Aldrich | Cat #V900408 |
| γ-aminobutyric acid (GABA) | Tocris | Cat #0344 |
| Ketanserin (Keta) | Aladdin | Cat #K107929 |
| Metoclopramide (Meto) | APExBIO | Cat #A3599 |
| SB216641 (SB) | APExBIO | Cat #B6653 |
| WAY-100635 (WAY) | Macklin | Cat #W855249 |
| Mineral oil | Sigma-Aldrich | Cat #69794 |
| 3-Octanol (OCT) | Sigma-Aldrich | Cat #218405 |
| 4-Methylcyclohexanol (MCH) | Sigma-Aldrich | Cat #153095 |
| Isoamyl acetate (IA) | Sigma-Aldrich | Cat #306967 |
| Fluoroshield | Sigma-Aldrich | Cat #F6182 |
| Fluoxetine | Sigma-Aldrich | Cat #F132 |
| Carbenoxolone (CBX) | Sigma-Aldrich | Cat #C4790 |
| CGP54626 (CGP) | Tocris | Cat #1088 |
| Picrotoxin (PTX) | Tocris | Cat #1128 |
| Experimental models: Organisms/strains | ||
| Canton-S (W1118) | Yi Rao, Peking University | N/A |
| Trh01 (Trhn−/−) | Qian et al.59 | N/A |
| 5-HT1AGal4 (5-HT1A−/−) | Qian et al.59 | N/A |
| LexAop2-ACh3.0 (chr2) | Jing et al.56 | BDSC: 86551 |
| LexAop2-ACh3.0 (chr3) | Jing et al.56 | BDSC: 86552 |
| UAS-5-HT1.0 (chr2) | Wan et al.58 | BDSC: 90874 |
| LexAop2-5-HT1.0 (chr2) | Wan et al.58 | BDSC: 90876 |
| LexAop2-5-HT1.0 (chr3) | Wan et al.58 | BDSC: 90877 |
| MB247-LexA | Yi Zhong, Tsinghua University | N/A |
| R13F02-Gal4 | Yi Rao, Peking University | BDSC: 48571 |
| R13F02-LexA | Yi Rao, Peking University | BDSC: 52460 |
| 30y-Gal4 | Yi Rao, Peking University | BDSC: 30818 |
| VT064246-Gal4 | Yi Rao, Peking University | VDRC: 204311 |
| UAS-CsChrimson-mCherry | Chuan Zhou, Institute of Zoology, CAS | BDSC: 82181 |
| UAS-Kir2.1 | Chuan Zhou, Institute of Zoology, CAS | N/A |
| UAS-hM4Di | Donggen Luo, Peking University | N/A |
| LexAop2-G-Flamp1 (chr2) | Wang et al.85 | N/A |
| UAS-5HT1A-RNAi | Jianquan Ni, TsingHuafly center | THU1216 |
| UAS-Gad1-RNAi | Yan Li, Institute of Biophysics, CAS | BDSC: 28079 |
| UAS-Trhn | Bloomington Drosophila Stock Center | BDSC: 27638 |
| R12G04-LexA | Bloomington Drosophila Stock Center | BDSC: 52448 |
| LexAop2-GCaMP6s (chr3) | Bloomington Drosophila Stock Center | BDSC: 44274 |
| UAS-GCaMP5 (chr2) | Bloomington Drosophila Stock Center | BDSC: 42037 |
| C316-Gal4 | Bloomington Drosophila Stock Center | BDSC: 30830 |
| L0111-lexA | Yi Rao, Peking University | N/A |
| LexAop2-CsChrimson.tdTomato | Bloomington Drosophila Stock Center | BDSC: 82183 |
| GH146-Gal4 | Bloomington Drosophila Stock Center | BDSC: 30026 |
| UAS-GCaMP5 (chr3) | Bloomington Drosophila Stock Center | BDSC: 42038 |
| Software and algorithms | ||
| Origin2019 | OriginLab | https://imagej.nih.gov/ij/; RRID: SCR_003070 |
| ImageJ | NIH | https://imagej.nih.gov/ij/; RRID: SCR_003070 |
| Arduino UNO | Arduino.cc | https://www.arduino.cc/en/Guide/ArduinoUno; RRID:SCR_017284 |
| Matlab R2019b | MathWorks | https://www.mathworks.com/; RRID:SCR_001622 |