FIG. 2.

Relocalization of p53 but not p51 by Ad5 E1B-55 kDa. H1299 cells (a human cell line lacking p53) were transiently transfected with plasmids (100 ng) expressing HA-tagged p53 or p51A. In addition, expression plasmids (400 ng) for FLAG-tagged Ad5 E1B-55 kDa or FLAG-tagged bacterial alkaline phosphatase (BAP) were cotransfected as indicated. The cells were fixed and stained simultaneously with murine anti-HA antibody and rabbit anti-FLAG antibody, followed by secondary antibodies labeled with different fluorescent dyes. The same areas were visualized for expression of HA-tagged (a to d) and FLAG-tagged (e to h) proteins. In addition, the cells were stained with DAPI (i to l) to show the location of cell nuclei.