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Reduction of p53 levels by adenovirus oncoproteins in murine cells. NIH 3T3 cells were transfected with expression plasmids for HA-tagged p53, Ad12 (A) or Ad5 (B) E1B-55 kDa (300 ng), and E4orf6 (900 ng) or the corresponding vector constructs, as indicated below the lanes. At 24 h after transfection, the cells were harvested, and p53 was detected by Western blotting with an antibody to the HA tag.
