Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 21;29(3):566–579. doi: 10.1038/s41380-023-02348-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Schematic representation of the assembloid experimental design. Differentiation protocols were performed in parallel prior to combining cells for assembloid generation. b Expression density of selected markers on Uniform Manifold Approximation and Projection (UMAP) plots showing integrated scRNA-Seq from 3 libraries of assembloids composed of control organoids and control microglia and 2 libraries of assembloids composed of control organoids and TREX1-KO microglia (in total, 54,192 cells). c Cell annotations on UMAP plot based on scRNA-seq data. d Left: Percentage distribution of cell types by conditions. Center: Fold change (log2) of percentage distribution between microglia genotype. Right: As in left but showing only unique fractions by microglia genotype. Cells color-coded in all graphs as in (left). The largest difference (by fold change) between microglia genotypes corresponds to oligodendrocyte cell types (MOLs, dividing OPCs, OPCs > t-OPCs > pre-OPCs). The largest differences (by quantity) correspond to a gain of excitatory and astroglia populations and a loss of inhibitory subtypes with mutant microglia. Radial glia with neuronal fate shifted to radial glia with glial fate. e Re-analysis of oligodendrocyte lineage and astroglia on UMAP plot with annotated cell types as indicated. f Expression density of selected markers. g Pseudotime analysis of oligodendrocyte lineage and astroglia starting at pre-OPCs. h Dot plot showing expression of selected marker genes in the five subpopulations of the oligodendrocyte lineage and astroglia. Dot sizes are the percentages of cells in each subpopulation that have detectable expression for the corresponding gene. Most relevant markers highlighted: EGFR primarily for pre-OPC and t-OPC; DLL3 primarily for t-OPC; PDCH15 for OPCs; PDGFRA for OPCs except t-OPCs. All cells express OLIG1. Dot color intensity represents average gene expression. MG: microglia; Oligo: oligodendrocyte; Astro: astrocyte; Micro: microglia; Exc.: excitatory; Inh.: inhibitory; Div.: dividing; RG: radial glia; IPC: intermediate progenitor cells; ChP: choroid plexus; OPC: oligodendrocyte progenitor cells; MGE: medial ganglionic eminence; CGE: caudal ganglionic eminence; LGE: lateral ganglionic eminence; PSCs: pluripotent stem cells.