Fig. 3.

Effect of H2AX deletion on anaphase bridge formation and blastocyst development. A: Immunostaining for H2AX in wild type (WT) and H2AX-deleted (H2AX-KO) zygotes. Scale bar, 20 μm. B: Effect of H2AX deletion on anaphase bridge formation. The cumulative results from three independent experiments are shown. In total, 56 WT and 57 H2AX-KO nonirradiated zygotes, and 85 WT and 96 H2AX-KO irradiated zygotes were examined. Fisher’s exact test was used for statistical analysis (ns, not significant; **** P < 0.0001). C: Effect of H2AX deletion on blastocyst development. The cumulative results from three independent experiments are shown. In total, 70 WT and 52 H2AX-KO nonirradiated zygotes, and 58 WT and 49 H2AX-KO irradiated zygotes were examined. Fisher’s exact test was used for statistical analysis (ns, not significant; * P < 0.05).