Fig. 1. CCHFV infections in Ldlr-knockout cells.
a, Levels of infection in control wild-type AN3-12 haploid and sister knockout (KO) cells infected with VSV, VSV-CCHF_G, CCHFV IbAr10200 and RVFV (MOI 0.1, 48 h post infection (h.p.i.)). Level of infection was assessed by RT–qPCR for viral and RNase P RNA. b, Levels of infection of IbAr10200 CCHFV in wild-type (WT) and two different LDLR KO (clones C2 and C12) Vero cells and c, in three different clones of LDLR KO (clones C8, C10 and C11) A549 cells. d, Levels of infection of RVFV in wild-type and two different LDLR KO (clones C2 and C12) Vero cells and e, in three different clones of LDLR KO (clones C8, C10 and C11) A549 cells. All mutant clones in b–e were generated using CRISPR/cas9 (Extended Data Fig. 3). Mutant haploid clones were from our previously reported Haplobank. All infections of diploid cells were done at an MOI of 0.1 for 24 h. Data are mean ± s.d. of n = 3 independent experiments. P values were calculated using two-sided unpaired Student’s t-test (Fig. 2a) and one-way ANOVA (Fig. 2b–e). **P < 0.01, ***P < 0.001, NS P > 0.05. Exact P values are available in.