Extended Data Fig. 6. IGCR1 is a weaker anchor than Cer-Sis in preventing over-utilization of proximal deletional Vκs. Related to Fig. 4.
a-f, Relative utilization percentage of individual Vκs in Igh-Igκ hybrid-Vκ line (a), Igh-Igκ hybrid-Vκ-JκRSS line (b), single Jκ5-Cer KO line (c), single Jκ5 allele line (d), single Jκ5-Sis KO line (e), single Jκ5-CerSis KO line (f) analyzed with indicated baits. Bar graph in the inset of each panel shows the percentage of distal (blue), middle (gray) and proximal (orange) Vκ domain usage from the corresponding line. g, Percentage of pooled RAG off-target junctions in Igκ locus from single Jκ5 allele line. Right panel: zoom-in to the region between Cer and Jκ, highlighted in yellow. h, Percentage of inversional (red) and deletional (blue) cryptic RSS junctions from single Jκ5 allele line within indicated regions as in Fig. 2d. i-j, RAG off-target profiles in single Jκ5-CerSis KO line presented as in g-h. A group of aberrant pseudo-normal coding-end junctions48 to sequences near the Igκ downstream CBE were excluded4. The patterns of Vκ usage in a, b and c are highly similar (a and c, Two-sided Pearson’s r = 0.91, P = 1.5e-63; b and c, Two-sided Pearson’s r = 0.97, P = 1.0e-99). The data shown in a and d are the same as that shown in Extended Data Fig. 5d and Fig. 2f, the data shown in g and h are the same as that shown in Fig. 2g and h, respectively, plotted here for better alignment and comparison with other results. Vκ utilization and cryptic RSS data are presented as mean ± s.e.m. from 3 (a,b,e), 4 (c), 7 (d,h) or 6 (f,j) biological repeats.